This is an image of dog femur that has had a screw implant for orthopedic surgery. The image was to check the integrity of the implant after a year.

Cortical Bone Image

Dr. Felix Wehrli and Dr. Alex Wright, University of Pennsylvania

Micro-CT image volume acquired at 17 µm isotropic resolution using an eXplore Locus SP specimen scanner, revealing the pores in a cortical bone specimen from the midshaft of a rabbit tibia. Skeletal hypomineralization (osteomalacia) was induced via a low-phosphorus diet, which manifested as increased cortical porosity compared to normal control rabbits. (M. A. Fernandez-Seara, et al., presented at the 25th Annual Meeting of the American Society for Bone and Mineral Research, Minneapolis, 2003.)

The purpose of this study was to compare computational to experimental mechanical properties, and determine which processing parameters are most likely to affect fabricated scaffold mechanics. These images show micro CT rendering image (left) and micro CT image (right) of manufactured 70% porous scaffold showing defects.

Functional Bone Tissue Engineering

Scott J Hollister, University of Michigan
Collaborators include Lin, C-Y; Schek, RM; Mistry, AS; Shih, X; Mikos AG; Krebsbach;, PH, of the University of Michigan.
Presented at the 51st Annual Meeting of the Orthopaedic Research Society

In this study, the integration of topology optimized scaffolds, osteoconductive polymer composite, and ex vivo gene therapy was shown to produce a functional osteogenic construct for bone tissue engineering. This image shows cross sections with increasing bone tissue growth over 12 weeks.

Engineered Wavy Fibered Porous Soft Tissue Scaffolds

Scott J Hollister, University of Michigan
Collaborators include Saito, E; Partee, BI; Das, S, of the University of Michigan.
Presented at the 51st Annual Meeting of the Orthopaedic Research Society

To address the need for soft tissue scaffolds with controlled porosity and mechanical properties, a wavy fiber scaffold design was developed. This image shows the scaffold design (left), the micro CT rendering (middle) and the fabricated scaffold picture (right).

Bone Tissue Engineering Scaffolds

Jessica M Williams, University of Michigan
Collaborators include Adebisi Adewunmi, Rachel M Schek, Colleen L Flanagan, Paul H Krebsbach, Steven E Feinberg, Scott J Hollister and Suman Das of the University of Michigan.
Published in Biomaterials 26 (2005) 4817-27.

In this work, porous PCL scaffolds were computationally designed and then fabricated via selective laser sintering (SLS), a rapid prototyping technique. This image shows an isometric view of a surface rendering of the design file for the subcutaneous-size scaffold (a), bottom view (b), side view (c), top view of micro CT bone surface rendering data combined with a surface rendering of the design file (d). PCL scaffold is shown in blue and mineralized matrix is shown in white.

This image shows the top view of micro CT data slice (same orientation as d, above) and illustrates the cortical shell and areas of trabeculated structures within the marrow space.

• Top of the page

Bone Tumor in Mouse

Jonathan B. Moody, Molecular Imaging Research, Inc.
Collaborators include Richard Lister, Patrick McConville, Alnawaz Rehemtulla2, William L. Elliott, Wilbur R. Leopold and Erin Trachet, of MIR Preclinical Services, and Alnawaz Rehemutlla of University of Michigan, Department of Radiation Oncology.

Image shows two views of a volume rendering of a large lesion on the proximal tibia (arrowheads). Another smaller lesion is evident on the femur (arrow). In microCT data, bone lesions appear as holes with size approximately proportional to the size of the metastatic tumor. Image was presented on the poster ‘In vivo Imaging of a Mouse Model of PC-3 Bone Metastasis’ at the American Association for Cancer Research.

Metastasis in the right shoulder that was previously detected by Bioluminescence imaging were followed by serial microCT (arrows). Bone destruction was clearly evident as tumor progressed. The image was presented on the poster ‘In vivo Imaging of a Mouse Model of PC-3 Bone Metastasis’ at the American Association for Cancer Research.

In vivo uCT mouse liver tumor model using eXplore Locus Courtesy University of Wisconsin

Mouse tumor model and 3D rendering using eXplore Locus. Courtesy of the University of Wisconsin

• Top of page